Tandem Labs

MarkerScan^SM for Biomarker Discovery and Screening

Targeted Biomarker Assays

Tandem Labs offers targeted LC/MS/MS assays of specific biochemical molecules to evaluate a compound's potential as a surrogate marker of drug safety and/or efficacy, either discretely or in panels.

Targeted Biomarker Assay Level 1

• Single marker or a set of marker panel
• Relatively quantitative analysis (no standard calibration curve)

Targeted Biomarker Assay Level 2

• Single marker or a set of marker panel
• Quantitative analysis (front/back standard calibration curve)

Targeted Biomarker Assays

Service Category	Applicable	Approach	Assay Expectation	Deliverables	Utilities
Targeted Biomarker Assay Level I	• Target validation • Lead optimization • Preclinical • Clinical • Post Market	LC/MS/MS	• Single or multiple known markers • Biochemical profiling (relative quantification) • No standard calibration • QC: one level (n=5, front/back)	Identify markers of interest	• Support target validation • Assist compound selection • Support PK/PD modeling • Help dose projection • Assist toxicity evaluation
Targeted Biomarker Assay Level II	• Target validation • Preclinical • Clinical • Post Market	LC/MS/MS	• Single or multiple known markers • LC/MS/MS quantification • Standard calibration (front/back curve) • QC: three levels	Determine concentrations of markers	Use markers for: • Disease diagnostics • Toxicity evaluation • Efficacy measurement • Patient selection • Adverse effect monitoring

MarkerScan^SM: Biomarker Discovery

MarkerScan^SM is a biomarker discovery and screening informatics process that efficiently identifies potential biomarkers derived from high mass resolution data obtained from two complimentary mass spectrometry platforms: MALDI/TOF/TOF and LC/QTOF.

Biomarker Discovery Level 1

• Biochemical profiling
• Discover potential biomarkers of interests

Biomarker Discovery Level 2

• Unknown biomarker identification

Biomarker Discovery

Service Category	Applicable	Approach	Assay Expectation	Deliverables	Utilities
Biomarker Discovery Level I	• Target validation • Preclinical • Clinical • Post market	LC/MS/MS	• Biochemical profiling • Relative quantitation • No standard calibration • QC: one level (n=5, front/back)	Descover potential markers of interest	Discovery of potential: • new targets • toxicity-related biomarkers • efficacy biomarkers • adverse effect biomarkers
		LC-TOF/MS
		MALDI-TOF/MS
Biomarker Discovery Level II	• Target validation • Preclinical • Clinical • Post market	LC/MS/MS	• Biochemical profiling • Relative quantitation • No standard calibration • QC: one level (n=5, front/back) • Biomarker ID	Discover potential markers of interest	• New therapeutic targets • Clinical diagnostics • Understand mechanisms of action • Convert to targeted biomarker assays
		LC-TOF/MS		Identify unknown markers of interest
		MALDI-TOF/MS